This dataset comprises hydrographic data from novel sensors on underwater autonomous gliders, to be used to characterise the physical, biological and chemical marine environment of the Mediterranean Sea (NOSUG project). Gliders were deployed in the Mediterranean Sea during Spring 2016 at the BOUSSOLE/DyFAMed sites by the University of East Anglia. Variables in the dataset include sea water temperature, salinity, pressure, dissolved oxygen content, chlorophyll concentration, optical backscatter coefficients and pH. The aim was to test two experimental ISFET pH-pCO2 sensors and to estimate net community production. The data collection was jointly funded by the Defence Science and Technology Laboratory and the University of East Anglia.